Quantitative PCR
Quantitative PCR is a powerful, highly sensitive technique that can be
used to quantitate gene expression, determine gene copy number, detect
SNPs, and detect DNA from viral and bacterial microorganisms.
The Q- PCR Service uses the very sensitive, accurate and reproducible quantitative
PCR technique to measure the levels of gene expression by a Relative Quantitation
approach. The typical application is in validation of data coming from Affymetrix
experiments or in comparison of the RNA levels between two or more different
systems.
SNP analysis is based on the Assay-on-Demand from Applied Biosystem which is
a highly flexible technology for detection of SNPs. More than 160,000 validated
assays, 30,000 gene-coding-region assays, and approximately 1.8 million pre-designed,
genome-wide assays may be selected from a library of human assays. On top of
this, custom SNP Genotyping Assays can be created for any genome.
AROS utilizes Applied Biosystems 7000 platform to perform Q- PCR experiments.
Our 7000 system is equipped with 96-well blocks to facilitate high throughput
analysis. We support SybrGreen, Exiqon and Taqman reaction chemistries. With
Exiqon /Taqman chemistry, a labeled probe is included in the reaction to enhance
the specificity and sensitivity of target sequence detection. With SyberGreen
chemistry, amplified product is detected by its interaction with the SyberGreen
fluorescent dye, which selectively binds to double-stranded DNA templates. SyberGreen
chemistry is less expensive, but generally requires more effort to optimize reactions.
Although the Exiqon and Taqman chemistries are more expensive, they generally
provide better specificity without the need for extensive optimization. We also
offer primer and probe design services.