DNA extraction from Cells and Tissue
DNA Extraction
AROS has implemented Standard Operation Procedures (SOP’s) for
DNA extraction from tissues and cells. Reagents employed at AROS are
mainly TRIzol reagents from Invitrogen, and column based technologies
from Qiagen Inc. Tissue specimens are homogenized in Qiagen Tissuelyzer
homogenizer. AROS recommend its own in-house SOP’s for DNA isolation,
or use methods specifically requested by the customer.
Quantitation / Quality Analysis
RNA quantity is determined on a Nanodrop (ND-1000 Spectrophotometer)
by measuring optical density at A260 nm wavelength. The A260/A280
nm wavelength ratio is accessed and should be 1.8 – 2.0 for
high quality DNA preparations. Each sample is checked using Agarose
gel chromatography and all results are returned as part of our
Quality Control reporting process.
Sample storage
Following extraction and purification, the RNA is stored at -80°C. AROS offers
to bank the samples within AROS for a period of 6 months free of charge, or for
an extended period see Sample Banking
Reporting
The Investigator is provided with a printout and electronic file including the
A260/A280 nm wavelength ratio, the DNA concentration, volume and total yield.
The customers own standard report scheme may be provided to AROS for reporting.
Labels for Containers
“All sample tubes must be unambiguously labeled with information that allows
cross reference to a sample log sheet that should be e-mailed to AROS. For labeling
we recommend using barcodes or a permanent marker.
DNA extraction from Formalin-fixed, Paraffin embedded Tissue Sections
DNA Extraction
AROS has implemented Standard Operation Procedures (SOP’s) for
DNA extraction from Formalin-fixed, Paraffin embedded Tissue Sections.
Reagents employed at AROS for this is Puregene DNA purification
system from Gentra Systems. The yield of DNA is typically 1µg per
quarecentimeter of a 10µ tissue section but is highly dependent
on the condition used for fixation and the age of the paraffin
block.